For this article, we developed a method to standardise root meristem segmentations to allow for quantitative comparisons:

(A-C) Illustration of the straightening of root meristem 3D-meshes for examples of the different genotypes, before and after realignment. (D-F) Transversal and horizontal virtual sections of simplified root meristem models obtained by re-orientation (straightening) of 3D-meshes along the defined central and radial axes. Cells are shown as ellipsoids with semi-axis proportional to the cell length in the corresponding direction. Cells of the central xylem pole are marked by orange dots, of the QC by red dots, and of the xylem axis by blue dots. For each root, the central xylem pole was re-aligned with the vertical z-axis, the xylem axis with the radial y-axis, and the phloem poles with the radial x-axis. The original cellular parameters were maintained as cells were re-oriented along the respective axis.